The smart Trick of ceesdekker That No One is Discussing

The smart Trick of ceesdekker That No One is Discussing

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Employing single-molecule optical and AFM imaging and drive-spectroscopy approaches, we find to unravel the mechanistic elements of the structure and dynamics of chromosomes. Our exploration passions consist of:

It absolutely was only within the nineties though, when nanotechnology had advanced into a amount in which one molecules might be manipulated, that the 1st experimental units commenced to appear.

Multicolor imaging from the bacterial nucleoid and mobile division proteins with blue, orange, and in the vicinity of-infrared fluorescent proteins

Considering the fact that 2000, Dekker has shifted the principle focus of his perform toward biophysics where he scientific studies the Houses of one biomolecules and cells using the resources of nanotechnology. This modification of industry was driven by his fascination for your impressive operating of Organic molecular buildings, as well as through the prolonged-time period standpoint that a lot of exciting discoveries is often envisioned in this field.[5] Present traces of analysis in his biophysics group are while in the regions of:[6]

This yielded a breakthrough. Inside only some months, we had been ready to measure transportation by means of somebody single-walled carbon nanotube. Based on lower-temperature experiments by a brilliant PhD scholar in my group, Sander Tans (now a valued colleague and professor at Delft University of Technological know-how using an Lively study lab at AMOLF in Amsterdam), we confirmed that carbon nanotubes were real quantum wires with pretty extended (micrometres) Digital coherence lengths — confirming theoretical predictions of metallic behaviour.

Microbiology has only existed for your century, since we started to study micro-organisms much more critically. I believed nanobiology was an proper expression, mainly because all molecular Organic applicable interactions occur at the nanoscale: a protein is a few nanometres in size, DNA is two nm thick.’

All parameters had been precisely the same for every panel With https://www.ceesdekker.net this online video, except for the initial placement on the 6hb rod (proven in blue) around the nanopore. Simulations that terminated early due to translocation of your rod in the pore are marked by a grey background.

Learning DNA loop extrusion by SMC proteins for chromosome architecture with one-molecule assays.

2003, shown the 1st biosensors created outside of a carbon nanotube; settled the structure and system of DNA restore proteins; and discovery of a different approach for fabricating solid-condition nanopores for DNA translocation

Bulk-floor coupling identifies the mechanistic relationship concerning Min-protein patterns in vivo and in vitro

FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in bacterial cell division

2006, initially demonstration of molecular sorting inside a lab on the chip working with biomotors; discovery of nanobubbles in strong-state nanopores; and 1st estimate of electrokinetic energy conversion inside a nanofluidic channel

Visualization of unstained DNA nanostructures with advanced in-concentrate transmission electron microscopy strategies

Reliable-condition nanopores have established being a amazingly multipurpose probe for one-molecule Investigation of DNA. I'll explain several of our modern results – exclusively DNA knots – and also our attempts to increase the capabilities of strong-state nanopores even further, during the way of one-protein detection, graphene nanopores, plasmonic nanopores, and DNA origami nanopores.